| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Ezakiella coagulans | GCACGAAGTCGCTAACGGA | GCGAGCGGTGACAAGATTTC | 60.44 | 59.90 | 278 | 129 |
100.00%
|
100.00%
|
| Primer 22 | Ezakiella coagulans | AGTGCCGAGCCACGAAAT | TTGCAAGTTCGTCGGCAC | 59.65 | 58.98 | 209 | 128 |
100.00%
|
100.00%
|
| Primer 23 | Ezakiella coagulans | GCGATGGATGATTTAGCCGAA | CCCTTTGATTCCGGAGTAAGC | 58.86 | 58.71 | 218 | 128 |
100.00%
|
100.00%
|
| Primer 24 | Ezakiella coagulans | AGCGTGTTGGGGACGAAA | ACAAGCCCTTCGGAGGAA | 59.49 | 58.10 | 172 | 128 |
100.00%
|
100.00%
|
| Primer 25 | Ezakiella coagulans | CGCGTGAATGTTGATGGCA | TCCGACATTTCGCACGGT | 59.50 | 59.66 | 228 | 128 |
100.00%
|
100.00%
|
| Primer 26 | Ezakiella coagulans | ACGCGTGAATGTTGATGGC | TCCGACATTTCGCACGGT | 59.50 | 59.66 | 229 | 128 |
100.00%
|
100.00%
|
| Primer 27 | Ezakiella coagulans | CAAGGGCATCGTGCTTTCC | TCCGACATTTCGCACGGT | 59.49 | 59.66 | 154 | 128 |
100.00%
|
100.00%
|
| Primer 28 | Ezakiella coagulans | AAGGGCATCGTGCTTTCCC | TCCGACATTTCGCACGGT | 60.68 | 59.66 | 153 | 128 |
100.00%
|
100.00%
|
| Primer 29 | Ezakiella coagulans | CGGAATCATGACCGGAGCT | CGCCTTTGAAAGCACGCA | 59.56 | 59.67 | 191 | 128 |
100.00%
|
100.00%
|
| Primer 30 | Ezakiella coagulans | TGCAGGCTTAACACCGGT | CCGTGCCAATTGCCCAAA | 59.17 | 59.26 | 170 | 128 |
100.00%
|
100.00%
|